Molecular biology method

Tandem Affinity Purification

A technique used to isolate specific interacting partners of MACROMOLECULAR COMPLEXES that have been assembled with a tagged component (bait) that interacts with other partners in the complex. The interacting bait component is labeled with a dual-affinity tag that allows for capture of the complex by IMMUNOPRECIPITATION, followed by purification of only the partners of the complex interacting with the bait by affinity chromatography.